Composite

Part:BBa_K5159026:Design

Designed by: Tianyue WU   Group: iGEM24_HKUST   (2024-10-01)


pCIT1-GFP-LIP2t


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 738
    Illegal XbaI site found at 1462
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2361
    Illegal XhoI site found at 796
    Illegal XhoI site found at 925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 738
    Illegal XbaI site found at 1462
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 738
    Illegal XbaI site found at 1462
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2144


Design Notes

To ensure the comparability, identical spacers and terminator settings as the pTEF-GFP-LIP2t cassette are applied.


Source

Please refer to the sources of each subparts.

References